Journal: bioRxiv
Article Title: Surface-modified measles vaccines encoding oligomeric, fusion-stabilized SARS-CoV-2 spike glycoproteins bypass measles seropositivity, boosting neutralizing antibody responses to omicron and historical variants
doi: 10.1101/2022.12.16.520799
Figure Lengend Snippet: (A) Isotype analysis of anti-SARS-CoV-2 spike antibodies. Serum samples from IFNAR −/− -CD46Ge mice vaccinated once (day 21) or twice (day 42) with MR-CoV-S6p312 were analyzed by ELISA for IgG1 and IgG2a antibody binding to SARS-CoV-2. Serum from mice vaccinated twice with purified SARS-CoV-2 Spike adjuvanted with alum was used as a control for the Th2-biased humoral response. (B) Cytokine production from the splenocytes of vaccinated mice. Splenocytes isolated from vaccinated mice were stimulated as indicated in , and cytokine secretion in the supernatant was analyzed by multiplex cytokine analysis. Dots represent individual animals, and horizontal bars and error bars are the mean ± SD. IL-1b lower limit of detection (LLOD): 1.45 pg/mL; IL-12 LLoD: 1.68 pg/mL; TNF-a LLoD 3.48 pg/mL; IFN-γ LLoD 2.19 pg/mL; GM-CSF LLoD: 3.20 pg/mL;IL-6 LLoD: 5.52 pg/mL; IL-5 LLoD: 2.19 pg/mL; IL-2 LLoD 1.88 pg/mL;IL-4 LLoD: 1.37 pg/mL; IL-13 LLoD: 2.86 pg/mL. Statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparison test (*, p<0.05; **, p<0.003; ***, p<0.0003; ****, p<0.0001).
Article Snippet: Supernatants were then analyzed for the expression of IFN-γ, IL-6, IL-18, GM-CSF, IL-1β, IL12p70, IL-13, IL-2, IL-4, TNF-α and IL-5 cytokines using a mouse cytokine 11-plex antibody bead kit (Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel, Cat No# EPX110-20820-901, Thermo Fisher).
Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Purification, Isolation, Multiplex Assay